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The 71st participated in the Peninsular Campaign of 1862, although it isn't clear whether Wistar was actually present; at the Battle of Seven Pines (May 31 and June 1) the regiment was led by its major, and during the Seven Days Battles (June 30 and July 1) commanded by its lieutenant colonel.
The 22nd Massachusetts became part of the Army of the Potomac and left their winter quarters on March 10, 1862 to participate in Maj. Gen. George B. McClellan's Peninsular Campaign.
After the failure of the Peninsular Campaign in the spring of 1862, the Tribune pressured President Abraham Lincoln into installing John Pope as commander of the Army of Virginia.
In the Civil War, he served as assistant engineer in the Army of the Potomac during the Peninsular campaign and in the northern Virginia campaign, and from July, 1864, to September, 1865, commanded as colonel, a regiment of veteran volunteer engineers.